DDX3 Antibody

Product Specifications

Background

DDX3 Antibody: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

NCBI Gene ID

1654

Swiss Prot

O00571

Accession Number

AAC34298

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Infectious Disease

Purification

DDX3 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (80%)

Buffer

DDX3 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DDX3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

DDX3 antibody will detect both DDX3 and DBY.

Symbol

DDX3X

Positive Control 2

Cat. No. 17-011 - Hep G2 Cell Slide

NCBI Official Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked

NCBI Organism

Homo sapiens

Background Reference 01

Linder P, Lasko PF, Ashburner M, et al. Birth of the D-E-A-D box. Nature 1989; 337:121-2.

Background Reference 02

Park SH, Lee SG, Kim Y, et al. Assignment of a human putative RNA helicase gene, DDX3, to human X chromosome bands p11.3-->p11.23. Cytogenet. Cell Genet. 1998; 81:178-9.