XBP-1 Antibody

Product Specifications

Background

XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

NCBI Gene ID

7494

Swiss Prot

P17861

Accession Number

P17861

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Chemokines & Cytokines

Purification

XBP-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1216 - PC-3 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (83%)

Buffer

XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

XBP-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

XBP1

Positive Control 2

Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 3

Cat. No. 17-011 - Hep G2 Cell Slide

NCBI Official Name

X-box binding protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.

Background Reference 02

Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.

Background Reference 03

Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.

Background Reference 04

Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94) : functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.