Beclin-1 Antibody

Product Specifications

Background

Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.

NCBI Gene ID

8678

Swiss Prot

Q14457

Accession Number

AAH10276

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Autophagy, Cancer

Purification

Beclin-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 - A431 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Chicken: (100%), Pig: (100%), Rat: (100%), Bovine: (100%)

Buffer

Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Beclin-1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

BECN1

Positive Control 2

Cat. No. 17-002 - A-431 Cell Slide

NCBI Official Name

Beclin 1, autophagy related

NCBI Organism

Homo sapiens

Background Reference 01

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.

Background Reference 02

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

Background Reference 03

Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.

Background Reference 04

Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91.