Mcl-1 Antibody

Product Specifications

Background

Mcl-1 Antibody: Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least three distinct isoforms designated Mcl-1L, Mcl-1S and Mcl-1ES. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.

NCBI Gene ID

4170

Swiss Prot

Q07820

Accession Number

NP_068779

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Neuroscience, Cancer

Purification

Mcl-1 Antibody is Ion exchange chromatography purified.

Positive Control

Cat. No. 1207 - Raji Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (81%), Rat: (79%)

Buffer

Mcl-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Mcl-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Detects isoforms Mcl-1L and Mcl-1S

Symbol

MCL1

Positive Control 2

Cat. No. 17-007 - Raji Cell Slide

NCBI Official Name

Myeloid cell leukemia sequence 1 (BCL2-related)

NCBI Organism

Homo sapiens

Background Reference 01

Edwards SW, Derouet M, Howse M, et al. Regulation of neutrophil apoptosis by Mcl-1. Biochem Soc Trans. 2004; 32:489-92.

Background Reference 02

Cuconati A, Mukherjee C, Perez D, et al. DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells. Genes and Dev. 2003; 17:2922-32.

Background Reference 03

Opferman JT, Letai A, Beard C, et al. Development and maintenance of B and T lymphocytes require antiapoptotic MCL-1. Nature 2003; 426:671-6.

Background Reference 04

Kozopas KM, Yang T, Buchan HL, et al. MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. Proc. Natl. Acad. Sci. USA 1993; 90:3516-20.

Other Product Names

Mcl-1 Antibody: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3