Caspase-4 Antibody

Product Specifications

Background

Caspase-4 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.

NCBI Gene ID

837

Swiss Prot

P49662

Accession Number

AAA86890

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer, Neuroscience, Inflammasomes

Purification

Caspase-4 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1306 - Human Spleen Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (73%)

Buffer

Caspase-4 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Caspase-4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Depending on cell lines or tissues used, other cleavage products may be observed.

Symbol

CASP4

Positive Control 2

Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3

Cat. No. 17-208 - A-20 Cell Slide

NCBI Official Name

Caspase 4, apoptosis-related cysteine peptidase

NCBI Organism

Homo sapiens

Background Reference 01

Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.

Background Reference 02

Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.

Background Reference 03

Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.

Background Reference 04

Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1 β -converting enzyme family of cysteine proteases. J. Biol. Chem. 1995; 270:15250-6.