Caspase-3 Antibody

Product Specifications

Background

Caspase-3 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9.

NCBI Gene ID

836

Swiss Prot

P42574

Accession Number

NP_004337

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

Caspase-3 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (100%), Rabbit: (100%), Rat: (100%), Mouse: (87%), Bovine: (92%)

Buffer

Caspase-3 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Caspase-3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

30 kDa

Fragment

IgG

Specificity

At least two isoforms of Caspase-3 are known to exist.

Symbol

CASP3

NCBI Official Name

Caspase 3, apoptosis-related cysteine peptidase

NCBI Organism

Homo sapiens

Background Reference 01

Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74

Background Reference 02

Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.

Background Reference 03

Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.

Background Reference 04

Slee EA, Adrain C, and Martin SJ. Serial killers: ordering caspase activation events in apoptosis. Cell Death Diff. 1999; 6:1067-74.

Other Product Names

Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3

Tested Applications

ELISA, IHC-P

Protein ID

14790119

Physical Properties

Liquid

Available Sizes

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