WNV Core Antibody

Product Specifications

Background

WNV Core Antibody: West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.

NCBI Gene ID

912267

Swiss Prot

P06935

Accession Number

NP_776011

Host

Rabbit

Reactivity

Virus

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Infectious Disease

Purification

WNV Core Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Buffer

WNV Core Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

WNV Core antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Applications Notes

WNV Core antibody can be used for the detection of the WNV Core protein in ELISA. It will detect 10 ng of free peptide at 1 μ g/mL.

Symbol

WNVgp1

NCBI Official Name

Anchored core protein C

NCBI Organism

West Nile virus

Background Reference 01

Gould LH and Fikrig E. West Nile virus: a growing concern. J. Clin. Invest. 2004; 113:1102-7.

Background Reference 02

Chu JJ, Rajamanonmani R, Li J, et al. Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein. J. Gen. Virol. 2005; 86:405-12.

Background Reference 03

Chu JJ and Ng ML. Interaction of West Nile virus with α v β 3 integrin mediates virus entry into cells. J. Biol. Chem. 2004; 279:54533-41.

Background Reference 04

Yang JS, Ramanathan MP, Muthumani K, et al. Induction of inflammation by West Nile Virus capsid through the caspase-9 apoptotic pathway. Emerg. Infect. Dis. 2002; 8:1379-84.