Bim Antibody

Bim Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Independent Antibody Validation in Cell lines (Figure 2) shows similar BIM expression profile in human and mouse cell lines detected by two independent anti-BIM antibodies that recognize different epitopes, 2065 against N-terminus domain and 3405 against central domain. BIM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2065 3405 KD validation (Figure 7, 9, 10) : Anti-BIM antibody (3405) specificity was further verified by BIM specific knockdown. BIM signal in 293, U937, Jurkat and U266 cells transfected with BIM siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs or shRNA. KD validation3405 Regulated expression validation (Figure 8) : BIM expression detected by anit-BIM antibodies (3405) was up-regulated by flavopiridol treatment, which was blocked by Cycloheximide in U266 cells. Regulated expression validation3405

Bim antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD) . Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD) . Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD) . 3405 only detect isoform EL.