IRF3 Antibody

Product Specifications

Background

IRF3 Antibody: Interferons (IFN) s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by Iκ B kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.

NCBI Gene ID

3661

Swiss Prot

Q14653

Accession Number

NP_001562

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Chemokines & Cytokines, Homeostasis, Inflammasomes

Purification

IRF3 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1225 - Ramos Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (79%)

Buffer

IRF3 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IRF3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

43, 47, 50 kDa

Fragment

IgG

Specificity

At least three isoforms of IRF3 are known to exist.

Symbol

IRF3

Positive Control 2

Cat. No. 1405 - Mouse Kidney Tissue Lysate

NCBI Official Name

Interferon regulatory factor 3

NCBI Organism

Homo sapiens

Background Reference 01

Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54

Background Reference 02

Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.