Bcl-2 Antibody

Product Specifications

Background

Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

NCBI Gene ID

596

Swiss Prot

P10415

Accession Number

AAH27258

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

3335

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain. Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3335 3337

Field of Research

Apoptosis, Autophagy, Cancer

Purification

Bcl-2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1288 - A20 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%), Chicken: (93%)

Buffer

Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Bcl-2antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD) . Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD) . Rat Bcl-2 has one isoform (236aa, 27kD) . 3337 can detect all isoforms of human, mouse and rat

Symbol

BCL2

Positive Control 2

Cat. No. 1305 - Human Kidney Tissue Lysate

Positive Control 3

Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 4

Cat. No. 1219 – MCF-7 Cell Lysate

Positive Control 5

Cat. No. 1208 – THP-1 Cell Lysate

NCBI Official Name

B-cell CLL/lymphoma 2

NCBI Organism

Homo sapiens

Background Reference 01

Lockshin et al. Cell Death Differ. 2000; 7:2-7.

Background Reference 02

Cory et al. Oncogene 2003; 22:8590-607.

Background Reference 03

Heiser et al. Exp. Geron. 2004; 39:1125-35.

Background Reference 04

Hockenbery et al. Nature 1990; 348:334-6.

Other Product Names

Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

20072668

Physical Properties

Liquid

Available Sizes

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