PHAP I Antibody

PHAP I Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Independent Antibody Validation in Cell lines (Figure 2) shows similar PHAP I expression profile in human, mouse and rat cell lines detected by two independent anti-PHAP I antibodies that recognize different epitopes, 3145 against C-terminus domain and 3151 against C-terminus different domain. PHAP I proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3145 3151 KD validation (Figure 6) : Anti-PHAP I antibody (3151) specificity was further verified by PHAP I specific knockdown. PHAP I signal in T47D cells transfected with PHAP I siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation3151 Regulated expression validation (Figure 7, 8, 9) : PHAP I expression detected by anit-PHAP I antibodies (3151) was up-regulated in breast cancer tissues of patients (Figure 7) and the breast cancer cell line (Figure 8) as well as in the Atxn1 KO mice (Figure 9) . Regulated expression validation3151

PHAP I antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human PHAP I has one isoform (249aa, 28.6kD) . Mouse PHAP I has one isoform (247aa, 28.5kD) and rat PHAP I also has one isoform (247aa, 28.6kD) . 3151 can detect all isoforms of human, mouse and rat.

Parrish. Regulation of Apoptosis Following Mitochondrial Cytochrome c Release. Department of Pharmacology and Molecular Cancer Biology, Duke University. PhD Thesis, 2010.

PHAP I Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32