PAK5 Antibody

Product Specifications

Background

PAK5 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK5 was initially identified as a kinase expressed primarily in brain that while possessing a kinase domain and GTPase binding domain similar to PAK4 and PAK6, is completely different from both. Expression of PAK5 in neural based cell lines resulted in neurite outgrowth suggesting that PAK5 may be involved in regulating the cytoskeletal changes necessary for promoting neurite outgrowth. Other experiments suggest that unlike the other PAKs, PAK5 may inhibit apoptosis by phosphorylating the Bcl-2 family member Bad.

NCBI Gene ID

57144

Swiss Prot

Q9P286

Accession Number

Q9P286

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Homeostasis, Neuroscience

Purification

PAK5 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1213 - T24 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (100%), Rat: (92%)

Buffer

PAK5 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PAK5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

PAK7

Positive Control 2

Cat. No. 1204 - K562 Cell Lysate

Positive Control 3

Cat. No. 17-004 - K-562 Cell Slide

NCBI Official Name

P21 protein (Cdc42/Rac) -activated kinase 7

NCBI Organism

Homo sapiens

Background Reference 01

Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7.

Background Reference 02

Dan C, Nath N, Liberto M, et al. PAK5, a new brain-specific kinase, promotes neurite outgrowth in N1E-115 cells. Mol. Cell. Biol. 2002; 22:567-77.

Background Reference 03

Cotteret S, Jaffer ZM, Beeser A, et al. p21-activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylated BAD. Mol. Cell. Biol. 2003; 23:5526-39.