PUMA Antibody

Product Specifications

Background

PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1) . The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2) . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

NCBI Gene ID

27113

Swiss Prot

Q96PG8

Accession Number

NP_055232

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

3041

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation (Figure 2) shows similar PUMA expression profile in both human and mouse cell lines detected by two independent anti-PUMA antibodies that recognize different epitopes, 3041 against C-terminus and 3043 against the N-terminus. PUMA proteins are detected in most of the cell lines with different expression levels by the two independent antibodies. Independent Antibody Validation3041 3043 siRNA Knockdown Validation (Figure 3) : Anti-PUMA antibody (3041) specificity was further verified by PUMA specific siRNA knockdown. PUMA signal in HeLa cells transfected with PUMA siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA Knockdown Validation3041

Field of Research

Apoptosis, Neuroscience, Autophagy, Cancer

Purification

PUMA Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1210 - 293 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (100%)

Buffer

PUMA Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PUMA antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

23 kDa

Fragment

IgG

Specificity

A lower band at approximately 16 kDa was detected in Daudi and K562 cells, which may represent the PUMA isoform 2.

Isoform

Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD), isoform 2 (131aa, 14kD), isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD) . This antibody detects human isoform 1&2, mouse and rat PUMA (193aa, 21kD for both of them) .

Symbol

BBC3

Positive Control 2

Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 3

Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 4

Cat. No. 1204 - K562 Cell Lysate

NCBI Official Name

BCL2 binding component 3

NCBI Organism

Homo sapiens

Background Reference 01

Nakano and Vousden. Mol Cell. 2001; 7 (3) 683-94

Background Reference 02

Han et al. Proc Natl Acad Sci U S A. 2001;98 (20) :11318-23.

Citation 01

21353614

Citation 02

20404322

Citation 03

25057207

Citation 04

27056669

Citation 05

26470728

Citation 06

26247726

Citation 07

17297446

Citation 08

28427210

Other Product Names

PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3

Tested Applications

ELISA, WB, ICC, IF

Protein ID

15193488

Physical Properties

Liquid

Available Sizes

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