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EndoG Antibody

Product Specifications

Background

EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

NCBI Gene ID

2021

Swiss Prot

Q14249

Accession Number

NP_004426

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

3035

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KD validation (Figure 13, 14) : Anti-EndoG antibody (3035) specificity was further verified by EndoG specific knockdown. EndoG signal in human cancer cell lines transfected with EndoG siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation3035 Localization validation (Figure 7, 8, 10, 11) : EndoG translocation was observed in different cell lines and tissues after treatment. From mitochondria to cytoplasm: figure 7 and figure 8. From cytosol to nucleus: figure 10 and figure 11. Localization validation

Field of Research

Apoptosis, Cancer

Purification

EndoG Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

1 mg/mL

Buffer

EndoG Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

EndoG antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=3035

Fragment

IgG

Isoform

Human EndoG has only one isoform (297aa, 33kD) . Mouse EndoG has one isoform (294aa, 32kD) and Rat EndoG also has one isoform (294aa, 32kD) . 3035 can detect human, mouse and rat isoform.

Symbol

ENDOG

Positive Control 2

Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 3

Cat. No. 1307 - Human Pancreas Tissue Lysate

Positive Control 4

Cat. No. 11-101 - Human Pancreas Tissue Slide

NCBI Official Name

Endonuclease G

NCBI Organism

Homo sapiens

Background Reference 01

Li et al. Nature 2001;412 (6842) :95-9.

Background Reference 02

Parrish et al. Nature 2001;412 (6842) :90-4.

Background Reference 03

Hengartner. Nature. 2001;412 (6842) :27, 29.

Background Reference 04

Widlak et al. J Biol Chem. 2001;276 (51) :48404-9.

Citation 01

18504456

Citation 02

16151635

Citation 03

17292393

Citation 04

16689664

Citation 05

20574921

Citation 06

17955488

Citation 07

19459857

Citation 08

19808980

Citation 09

16728972

Citation 10

16046141

Citation 11

17050806

Citation 12

25865938

Citation 13

18699742

Citation 14

19793916

Citation 15

21377520

Citation 16

28122282

Other Product Names

EndoG Antibody: Endonuclease G, mitochondrial, Endo G

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

53759134

Physical Properties

Liquid

Available Sizes

Curated Selection

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