Nudel Antibody

Product Specifications

Background

Nudel Antibody: Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration; phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.

NCBI Gene ID

81565

Swiss Prot

Q9GZM8

Accession Number

AAF97497

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction

Purification

Nudel Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (100%), Rabbit: (100%), Chicken: (73%)

Buffer

Nudel Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Nudel antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

NDEL1

Positive Control 2

Cat. No. 17-005 - Jurkat Cell Slide

NCBI Official Name

NudE nuclear distribution gene E homolog (A. nidulans) -like 1

NCBI Organism

Homo sapiens

Background Reference 01

Niethammer H, Smith DS, Ayala R, et al. NUDEL is a novel Cdk5 substrate that associates with LIS1 and cytoplasmic dynein. Neuron 2000; 28:697-711.

Background Reference 02

Liu Z, Steward R, and Luo L. Drosophila Lis1 is required for neuroblast proliferation, dendritic elaboration and axonal transport. Nat. Cell Biol. 2000; 2:776-83.

Background Reference 03

Gupta A and Tsai LH. Cyclin-dependent kinase 5 and neuronal migration in the neocortex. Neurosignals 2003; 12:173-9.

Background Reference 04

Liang Y, Yu W, Li Y, et al. Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. J. Cell Biol. 2004; 164:557-66.