TRIM28 Antibody

Product Specifications

Background

TRIM28 Antibody: TRIM28, also known as KAP-1, is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily and contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TRIM28 is a corepressor for Kruppel-associated box (KRAB) -domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TRIM28 acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. TRIM28 associates with both heterochromatin and euchromatin, causing gene silencing by both HP1 binding and histone deacetylation.

NCBI Gene ID

10155

Swiss Prot

Q13263

Accession Number

NP_005753

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

TRIM28 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1313 - Human Testis Tissue Lysate

Concentration

1 mg/mL

Buffer

TRIM28 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TRIM28 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Calculated Molecular Weight

Predicted: 92 kDa

Fragment

IgG

Specificity

At least three isoforms of TRIM28 are known to exist; this antibody will detect all three isoforms

Symbol

TRIM28

NCBI Official Name

Tripartite motif containing 28

NCBI Organism

Homo sapiens

Background Reference 01

Friedman JR, Fredericks WJ, Jensen DE, et al. KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev. 1996; 10:2067-78.

Background Reference 02

Cammas F, Mark M, Dolle P, et al. Mice lacking the transcriptional corepressor TIF1beta are defective in early postimplantation development. Dev. 2000; 127:2955-63.

Background Reference 03

Chang CJ, Chen YL, and Lee SC. Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression. Mol. Cell Biol. 1998; 18:5880-7

Background Reference 04

Groner AC, Meylan S, Ciuffi A, et al. KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading. PLoS Genet. 2010; 6:e1000869.