IL-21 Antibody

IL-21 Antibody: A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

Independent Antibody Validation in Cell Lines (Figure 2) shows similar IL-21 expression profile in human and mouse cell lines detected by two independent anti-IL-21 antibodies that recognize different epitopes, 2465 against central domain and 2463 against C-terminus domain. IL-21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell Lines2465 2463 Recombinant Protein Test (Figure 4) : Anit-IL-21 antibodies (2465) detected human IL-21 recombinant protein. Recombinant Protein Test2465 Regulated expression validation (Figure 6, 7, 8) : IL-21 expression detected by anit-IL-21 antibodies (2465) was up down-regulated by WRT treatment (figure 7) or was up-regulated in Hp positive patients (figure 8) and in patients with Crohn’s disease (figure 6) . Regulated expression validation2465

IL-21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.

Human IL-21 has 2 isoforms, including isoform 1 (162aa, 18.7kD) and isoform 2 (153aa, 17.6kD) . Mouse IL-21 has one isoform (146aa, 16.8kD) and Rat IL-21 also has one isoform (146aa, 17.1kD) . 2465 can detect both human isoforms and also detect mouse and rat.