PERP Antibody

Product Specifications

Background

PERP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.

NCBI Gene ID

64058

Swiss Prot

Q9JK95

Accession Number

NP_071404

Host

Rabbit

Reactivity

Human

Immunogen

2451

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 1) shows similar PERP expression profile in human cell lines detected by two independent anti-PERP antibodies that recognize different epitopes, 2451 against C-terminus domain and 57-777 against C-terminus domain. PERP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2451 57-777

Field of Research

Apoptosis

Purification

PERP Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 - A431 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat (100%) ; Mouse (82%)

Buffer

PERP Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PERP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human PERP has one isoform (193aa, 21kD) . Mouse PERP has one isoform (193aa, 21.6kD) and Rat PERP also has one isoform (193aa, 21.6kD) . 2451 can detect human, mouse and rat.

Symbol

Perp

Positive Control 2

Cat. No. 17-002 - A-431 Cell Slide

NCBI Official Name

PERP, TP53 apoptosis effector

NCBI Organism

Mus musculus

Background Reference 01

Matsuoka et al. Science 1998; 282:1893-7.

Background Reference 02

Tanaka et al. Nature 2000; 404:42-9.

Background Reference 03

Oda et al. Science 2000; 288:1053-8.

Background Reference 04

Oda et al. Cell 2000; 102:849-62.

Other Product Names

PERP Antibody: THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik, Krtcap1, p53 apoptosis effector related to PMP-22, Keratinocyte-associated protein 1, KCP-1

Tested Applications

ELISA, WB, ICC

Protein ID

11528520

Physical Properties

Liquid

Available Sizes

Curated Selection

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