NAK Antibody

NAK Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. Phosphorylation of I-kappaB by Iκ B kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-κ B. The IKK complex contains IKKα , IKKβ , and IKKγ . A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-κ B-activating kinase), and T2K. NAK/TBK1 activates IKKβ through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κ B activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.

NAK antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Pomerantz JL, Baltimore D. NF-κ B activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J 1999;18 (23) :6694-704

Tojima Y, Fujimoto A, Delhase M, Chen Y, Hatakeyama S, Nakayama K, Kaneko Y, Nimura Y, Motoyama N, Ikeda K, Karin M, Nakanishi M. NAK is an Iκ B kinase-activating kinase. Nature 2000;404 (6779) :778-82

Bonnard M, Mirtsos C, Suzuki S, Graham K, Huang J, Ng M, Itie A, Wakeham A, Shahinian A, Henzel WJ, Elia AJ, Shillinglaw W, Mak TW, Cao Z, Yeh WC. Deficiency of T2K leads to apoptotic liver degeneration and impaired NF-κ B-dependent gene transcription. EMBO J. 2000;19 (18) :4976-85.