IKK gamma Antibody

IKK gamma Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. The Iκ B kinase (IKKα and IKKβ ) phosphorylates IkB and mediates NF-κ B activation. A novel molecule in the IKK complex was recently identified and termed IKKγ /NEMO/FIP3/IKKAP1. IKKγ interacts with IKKβ and is required for the activation of IKK complex and NF-κ B by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κ B activation.

IKK gamma antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a component of the Iκ B kinase complex essential for NF-κ B activation. Cell. 1998;93 (7) :1231-40.

Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A, Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a modulator of NF-κ B activity and as a target of an adenovirus inhibitor of tumor necrosis factor α -induced apoptosis. Proc Natl Acad Sci U S A 1999;96 (3) :1042-7

Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB, Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM. Iκ B kinase (IKK) -associated protein 1, a common component of the heterogeneous IKK complex. Mol Cell Biol. 1999;19 (2) :1526-38.