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IKK epsilon Antibody

Product Specifications

Background

IKK epsilon Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. Iκ B is phosphorylated by Iκ B kinase (IKK) complex that contains IKKα , IKKβ , and IKKγ . A novel molecule in the IKK complex was recently identified and designated IKKε /IKK-i. IKK epsilon is required for the activation of NF-κ B by PMA and T cell receptors but not by TNFα and IL-1. IKKε /IKK-i message is expressed in a variety of tissues and is inducible by TNFα , IL-1, and LPS.

NCBI Gene ID

9641

Swiss Prot

Q14164

Accession Number

AF241789

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

2329

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Induced Expression Validation (Figure 1) : Raw 264.7 cells were treated with LPS (0.3 µg /mL) for different time period (3 hrs and 6 hrs) . IKK epsilon expression detected by anit-IKK epsilon antibodies (2329) was induced by LPS treatment and the expression levels were increased gradually at 3 hrs and 6 hrs. Induced Expression Validation2329

Field of Research

Signal Transduction

Purification

IKK epsilon Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

1 mg/ml

Homology

Predicted species reactivity based on immunogen sequence: Rat: (75%)

Buffer

IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IKK epsilon antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=2329

Fragment

IgG

Specificity

It has no cross response to IKKa, IKKb, or IKKy.

Isoform

Human IKK epsilon has 2 isoforms, including isoform 1 (716aa, 80kD) and isoform 2 (631aa, 70kD) . Mouse IKK epsilon has one isoform (717aa, 81kD) and Rat IKK epsilon also has one isoform (717aa, 80kD) . 2329 can detect human, mouse and possibly rat.

Symbol

IKBKE

Positive Control 2

Cat. No. 1307 - Human Pancreas Tissue Lysate

Positive Control 3

Cat. No. 11-101 - Human Pancreas Tissue Slide

Positive Control 4

Cat. No. 1202 - A431 Cell Lysate

Positive Control 5

Cat. No. 1224 - Daudi Cell Lysate

NCBI Official Name

Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon

NCBI Organism

Homo sapiens

Background Reference 01

Peters et al. Mol Cell 2000;5 (3) :513-22.

Background Reference 02

Shimada et al. Int Immunol 1999;11 (8) :1357-62 (WD0101) .

Other Product Names

IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

7288877

Physical Properties

Liquid

Available Sizes

Curated Selection

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