ASC Antibody

Product Specifications

Background

ASC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4) . Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

NCBI Gene ID

29108

Swiss Prot

Q9ULZ3

Accession Number

BAA87339

Host

Rabbit

Reactivity

Human

Immunogen

2287

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar ASC expression profile in human and mouse cell lines detected by two independent anti-ASC antibodies that recognize different epitopes, 2287 against C-terminus domain and 39-001 against full length human recombinant protein. ASC proteins are detected in some of tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2287 39-001

Field of Research

Apoptosis, Inflammasomes

Purification

ASC Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1209 - HL60 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse (57%), Rat (57%)

Buffer

ASC Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ASC antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human ASC has 3 isoforms, including isoform 1 (195aa, 22kD), isoform 2 (176aa, 20kD), and isoform 3 (135aa, 15kD) . Mouse ASC has one isoform (193aa, 22kD) and rat ASC also has one isoform (193aa, 22kD) . 2287 can detect all human isoforms.

Symbol

PYCARD

Positive Control 2

Cat. No. 17-009 - HL-60 Cell Slide

Positive Control 3

Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 4

Cat. No. 1208 – THP-1 Cell Lysate

NCBI Official Name

PYD and CARD domain containing

NCBI Organism

Homo sapiens

Background Reference 01

Masumoto et al. J Biol Chem. 1999;274 (48) :33835-8.

Background Reference 02

Masumoto et al. Exp Cell Res. 2001;262 (2) :128-133.

Background Reference 03

Conway et al. Cancer Res. 2000;60 (22) :6236-42

Background Reference 04

McConnell et al. Cancer Res. 2000;60 (22) :6243-7. (WD0102)

Citation 01

15020601

Other Product Names

ASC Antibody: ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC