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RIP3 Antibody

Product Specifications

Background

RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κ B activation. Overexpression of RIP3 induces apoptosis and NF-κ B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

NCBI Gene ID

56532

Swiss Prot

Q9QZL0

Accession Number

AAF03133

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

2283

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KO Validation (Figure 7,9,13) shows RIP3 expression detected by anti-RIP3 antibodies (2283) was disrupted in multiple tissues of RIP3 KO mice (figure 7, 13) and in RIP3 KO MEF cells (figure 9) . KO Validation KD validation (Figure 10) : Anti-RIP3 antibody (2283) specificity was further verified by RIP3 specific knockdown. RIP3 signal in L929 cells transfected with RIP3 shRNA was disrupted in comparison with that in cells transfected with control shRNA. KD validation2283 Regulated expression validation (Figure 7,11,13) : RIP3 expression detected by anit-RIP3 antibodies (2283) was up-regulated by treatment of cerulein (figure 7), LPS (figure 11) or acetaminophen (figure 13) in different tissues or cells. Regulated expression validation2283 Overexpression validation (Figure 12) : RIP3 overexpression detected by anit-RIP3 antibodies (2283) was observed in three cell lines stably transfected with RIP3. Overexpression validation2283

Field of Research

Apoptosis

Purification

RIP3 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 – A431 Cell Lysate

Concentration

1 mg/ml

Buffer

RIP3 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

RIP3 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=2283

Fragment

IgG

Isoform

Mouse RIP3 has one isoform (486aa, 53kD) . Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57kD), isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD) . Rat RIP3 also has one isoform (478aa, 52kD) . 2283 can detect can detect isoforms of mouse and rat as well as human isoform 1.

Symbol

Ripk3

Positive Control 2

Cat. No. 1282 - 3T3/NIH Cell Lysate

Positive Control 3

Cat. No. 1285- C2C12 Cell Lysate

Positive Control 4

Cat. No. 1212- 3T3/Balb Cell Lysate

NCBI Official Name

Receptor-interacting serine-threonine kinase 3

NCBI Organism

Mus musculus

Background Reference 01

Yu et al. Curr Biol. 1999;9 (10) :539-42.

Background Reference 02

Sun et al. J Biol Chem. 1999;274 (24) :16871-5.

Background Reference 03

Pazdernik et al. Mol Cell Bio. 1999; 19 (10) :6500-8 (WD0102)

Citation 01

19524512

Citation 02

21368761

Citation 03

23201684

Citation 04

26943325

Citation 05

28981102

Citation 06

23744808

Citation 07

22817896

Citation 08

24441827

Citation 09

25326752

Citation 10

25939025

Citation 11

24012422

Citation 12

27321907

Citation 13

28085133

Citation 14

26418225

Citation 15

23734789

Citation 16

23348743

Citation 17

22262768

Citation 18

28871172

Citation 19

27532042

Citation 20

21402742

Other Product Names

RIP3 Antibody: Rip3, AW107945, 2610528K09Rik, Rip3, RIP-like protein kinase 3, RIP-3

Tested Applications

ELISA, IF, IHC-P, WB, IP

Protein ID

6063101

Physical Properties

Liquid

Available Sizes

Curated Selection

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