F1A alpha Antibody

Product Specifications

Background

F1A alpha Antibody: Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1β . F1Aalpha/FEM1β is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.

NCBI Gene ID

10116

Swiss Prot

Q9UK73

Accession Number

AAF05314

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

F1A alpha Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1404 - Mouse Liver Tissue Lysate

Concentration

1 mg/ml

Homology

Predicted species reactivity based on immunogen sequence: Chicken: (79%)

Buffer

F1A alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

F1A alpha antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

70 kDa

Fragment

IgG

Symbol

FEM1B

Positive Control 2

Cat. No. 1464 - Rat Liver Tissue Lysate

NCBI Official Name

Fem-1 homolog b (C. elegans)

NCBI Organism

Homo sapiens

Background Reference 01

Chan SL, Tan KO, Zhang L, Yee KS , Ronca F, Chan MY, Yu VC. F1Aα , a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274 (45) :32461-8.

Background Reference 02

Ventura-Holman T, Maher JF. Sequence, organization, and expression of the human FEM1B gene. Biochem Biophys Res Commun 2000;267 (1) :317-20

Background Reference 03

Chan SL, Yee KS , Tan KM, Yu VC. The Caenorhabditis elegans sex determination protein FEM-1 is a CED-3 substrate that associates with CED-4 and mediates apoptosis in mammalian cells. J Biol Chem. 2000;275 (24) :17925-8. (WD0102)