Nephrin Antibody

Product Specifications

Background

Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.

NCBI Gene ID

4868

Swiss Prot

O60500

Accession Number

NP_004637

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

2265

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Induced Expression Validation (Figure 10) : Nephrin expression detected by anit-Nephrin antibodies was up-regulated by curcumin treatment in type 1 diabetic rats. Induced Expression Validation Localization Validation (Figure 11) : Nephrin detected by anti-Nephrin antibodies is colocalized with PKGIα at the tips of the cell processes in rat podocytes. Localization Validation Regulated expression validation (Figure 14) : Nephrin expression detected by anit-Nephrin antibodies was down-regulated by PEGF treatment (NGP or HGP), which was reversed by the addition of C3 transferase in mouse podocyte cells. Regulated expression validation

Field of Research

Homeostasis

Purification

Nephrin Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1405 - Mouse Kidney Tissue Lysate

Concentration

1 mg/mL

Buffer

Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Nephrin antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD) . Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD) . Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD) . 2265 can detect isoforms of human, mouse and rat.

Symbol

NPHS1

Positive Control 2

Cat. No. 1203 - A549 Cell Lysate

Positive Control 3

Cat. No. 1285 - C2C12 Cell Lysate

NCBI Official Name

Nephrosis 1, congenital, Finnish type (nephrin)

NCBI Organism

Homo sapiens

Background Reference 01

Kestila et al. Mol. Cell 1998; 1:575-582.

Background Reference 02

Tryggvason. J. Am. Soc. Nephrol. 1999; 10:2440-5.

Background Reference 03

Tryggvason and Wartiovaara. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.

Citation 01

28194887

Citation 02

26151763

Citation 03

24004644

Citation 04

22898567

Citation 05

23454089

Citation 06

22212473

Citation 07

30536192

Citation 08

21520075

Citation 09

29032153

Other Product Names

Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

4758822

Physical Properties

Liquid

Available Sizes

Curated Selection

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