What is the catalog number for Nephrin Antibody?

The catalog number for Nephrin Antibody is 2265.

What sizes are available for Nephrin Antibody?

Nephrin Antibody is available in the following size(s): 0.02 mg, 0.1 mg.

How many pack sizes does Nephrin Antibody come in?

Nephrin Antibody is available in 2 different pack sizes.

Is Nephrin Antibody in stock?

Nephrin Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

Nephrin Antibody

Product Specifications

Background

Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.

NCBI Gene ID

4868

Swiss Prot

O60500

Accession Number

NP_004637

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

2265

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Induced Expression Validation (Figure 10) : Nephrin expression detected by anit-Nephrin antibodies was up-regulated by curcumin treatment in type 1 diabetic rats. Induced Expression Validation Localization Validation (Figure 11) : Nephrin detected by anti-Nephrin antibodies is colocalized with PKGIα at the tips of the cell processes in rat podocytes. Localization Validation Regulated expression validation (Figure 14) : Nephrin expression detected by anit-Nephrin antibodies was down-regulated by PEGF treatment (NGP or HGP), which was reversed by the addition of C3 transferase in mouse podocyte cells. Regulated expression validation

Field of Research

Homeostasis

Purification

Nephrin Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1405 - Mouse Kidney Tissue Lysate

Concentration

1 mg/mL

Buffer

Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Nephrin antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD) . Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD) . Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD) . 2265 can detect isoforms of human, mouse and rat.