Survivin Antibody

Product Specifications

Background

Survivin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.

NCBI Gene ID

332

Swiss Prot

O15392

Accession Number

NP_001159

Host

Rabbit

Reactivity

Human

Immunogen

2235

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar Survivin expression profile in human cell lines detected by two independent anti-Survivin antibodies that recognize different epitopes, 2233 against N-terminus domain and 2235 against C-terminus domain. Survivin proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2233 2235 Recombinant Protein Test (Figure 6- 8) : Anti-Survivin antibodies (2235) detected human Survivin recombinant protein directly or in sera from cancer patients. Recombinant Protein Test2235

Field of Research

Apoptosis, Cancer

Purification

Survivin Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1206 - MOLT4 Cell Lysate

Concentration

1 mg/ml

Homology

Predicted species reactivity based on immunogen sequence: Pig: (92%), Bovine: (79%), Mouse: (71%), Rat: (71%)

Buffer

Survivin Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Survivin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human Survivin has 7 isoforms, including isoform 1 (142aa, 16kD), isoform 2 (165aa, 19kD), isoform 3 (137aa, 16kD), isoform 4 (120aa, 14kD), isoform 5 (117aa, 14kD), isoform 6 (78aa, 9kD) and isoform 7 (74aa, 8.5kD) . Mouse Survivin has 3 isoforms, including isoform 1 (140aa, 16kD), isoform 2 (121aa, 14kD) and isoform 3 (40aa, 4.7kD) . Rat Survivin has one isoform (142aa, 17kD) . 2235 can detect human and possibly mouse and rat.

Symbol

BIRC5

Positive Control 2

Cat. No. 17-006 - MOLT-4 Cell Slide

Positive Control 3

Cat. No. 1201 - HeLa Cell Lysate

Positive Control 4

Cat. No. 1204 - K562 Cell Lysate

Positive Control 5

Cat. No. 1224 - Daudi Cell Lysate

NCBI Official Name

Baculoviral IAP repeat-containing 5

NCBI Organism

Homo sapiens

Background Reference 01

Ambrosini et al. Nat Med 1997;3:917-21.

Background Reference 02

Li et al. Nature 1998;396:580-4.

Background Reference 03

Kobayashi et al. Proc Natl Acad Sci USA 1999;96:1457-62 (WD0600) .

Citation 01

17067715

Citation 02

15896923

Citation 03

20523766

Other Product Names

Survivin Antibody: API4, EPR-1, API4, IAP4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4

Tested Applications

ELISA, WB, ICC, IF

Protein ID

59859878

Physical Properties

Liquid

Available Sizes

Curated Selection

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