DEDAF Antibody

Product Specifications

Background

DEDAF Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

NCBI Gene ID

23429

Swiss Prot

Q8N488

Accession Number

AF179286

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

DEDAF Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1203 - A549 Cell Lysate

Concentration

1 mg/mL

Buffer

DEDAF Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DEDAF antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

32 kDa

Fragment

IgG

Symbol

RYBP

Positive Control 2

Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 3

Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 4

Cat. No. 17-003 - A549 Cell Slide

NCBI Official Name

RING1 and YY1 binding protein

NCBI Organism

Homo sapiens

Background Reference 01

Zheng L, Schickling O, Peter ME, et al. The death effector domain-associated factor (DEDAF) plays distinct regulatory roles in the nucleus and cytoplasm. J. Biol. Chem. 2001; 276:31945-52.

Background Reference 02

Garcia E, Marcos-Gutierrez C, del Mar Lorente M, et al. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 1999;18:3404-18.