APP Antibody

Product Specifications

Background

APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99) . The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease.

NCBI Gene ID

351

Swiss Prot

P05067

Accession Number

CAA30050

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KO Validation (Figure 1) : Anti-APP antibodies (2133) specificity was further verified by APP specific knockout. APP signal was not detected in APP knockout 293T cells as compared to that in control wild type cells. KO Validation

Field of Research

Neuroscience

Purification

APP Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1403 - Mouse Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%), Rabbit: (100%), Guinea pig: (100%), Pig: (100%)

Buffer

APP Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

APP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

APP

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Amyloid beta (A4) precursor protein

NCBI Organism

Homo sapiens

Background Reference 01

Ponte P, Gonzalez-DeWhitt P, Schilling J, et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988; 331:525-7.