What is the catalog number for DFF40 Antibody?

The catalog number for DFF40 Antibody is 2107.

What sizes are available for DFF40 Antibody?

DFF40 Antibody is available in the following size(s): 0.02 mg, 0.1 mg.

How many pack sizes does DFF40 Antibody come in?

DFF40 Antibody is available in 2 different pack sizes.

Is DFF40 Antibody in stock?

DFF40 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

DFF40 Antibody

Product Specifications

Background

DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) . The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

NCBI Gene ID

13368

Swiss Prot

O54788

Accession Number

NP_031885

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

DFF40 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1204 - K562 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Buffer

DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DFF40 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

Dffb

Positive Control 2

Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3

Cat. No. 17-005 - Jurkat Cell Slide

NCBI Official Name

DNA fragmentation factor, beta subunit

NCBI Organism

Mus musculus

Background Reference 01

Enari M, Sakahira H, Yokoyama H, et al. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998; 391:43-50.

Background Reference 02

Sakahira H, Enari M, and Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998; 391:96-99.

Background Reference 03

Halenbeck R, MacDonald Human, Ratoulston A, et al. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr. Biol. 1998; 8:537-40.

Background Reference 04

Liu X, Li P, Widlak P, at al. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc. Natl. Acad. Sci. USA 1998; 95:8461-6.