DNase II Antibody

Product Specifications

Background

DNase II Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.

NCBI Gene ID

1777

Swiss Prot

O00115

Accession Number

AF047016

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Signal Transduction

Purification

DNase II Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1306 - Human Spleen Tissue Lysate

Concentration

1 mg/ml

Buffer

DNase II Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DNase II antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

Aprox: 40 kDa

Fragment

IgG

Symbol

DNASE2

Positive Control 2

Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 3

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

Deoxyribonuclease II, lysosomal

NCBI Organism

Homo sapiens

Background Reference 01

Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8

Background Reference 02

Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6