IKK alpha Antibody

IKK alpha Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. The long-sought Iκ B kinase (IKK), which phosphorylates Iκ B, and mediates Iκ B degradation and NF-κ B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ ) . IKKα and IKKβ interact with each other and both are essential for the NF-κ B activation. IKKα specifically phosphorylates IkB-alpha. IKKα is expressed in variety of human tissues.

Overexpression validation (Figure 8) : ASC expression detected by anit-ASC antibodies (2287) was shown in ASC transfected 293T cells, but not in WT 293T cells. Overexpression validation2287

IKK alpha antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive Iκ B kinase that activates the transcription factor NF-κ B. Nature 1997;388:548-54

Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The Iκ B kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ , necessary for Iκ B phosphorylation and NF-κ B activation. Cell 1997;91:243-52

Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. Iκ B kinase-β : NF-κ B activation and complex formation with Iκ B kinase-α and NIK. Science 1997;278:866-9

IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha