DR5 Antibody

Product Specifications

Background

DR5 Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-κ B.

NCBI Gene ID

8795

Swiss Prot

O14763

Accession Number

AF012535

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KO Validation (Figure 12) shows DR5 expression detected by anti-DR5 antibodies (2019) was disrupted in DR5 KO HCT116 cells. KO Validation2019 KD validation (Figure 13) : Anti-DR5 antibody (2019) specificity was further verified by DR5 specific knockdown. DR5 signal in MB231 cells transfected with DR5 siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation2019 Regulated expression validation (Figure 12, 15) : DR5 expression detected by anit-DR5 antibodies (2019) was up-regulated by carfilzomib treatment and was down-regulated by treatment with two or more cytokines. Regulated expression validation2019

Field of Research

Apoptosis

Purification

DR5 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/ml

Buffer

DR5 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DR5 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Antibody has no cross reaction to DR4.

Isoform

Human DR5 has 3 isoforms, including isoform L (440aa, 48kD), isoform S (411aa, 45kD) and isoform 3 (118aa, 13kD) . Mouse DR5 has 1 isoform (381aa, 42kD), and Rat DR5 also has 1 isoform (336aa, 37kD) . 2019 can detect two human isoforms and can also detect mouse and rat isoforms.

Symbol

TNFRSF10B

Positive Control 2

Cat. No. 1204 - K562 Cell Lysate

Positive Control 3

Cat. No. 17-001 - HeLa Cell Slide

Positive Control 4

Cat. No. 1282 - 3T3/NIH Cell Lysate

Positive Control 5

Cat. No. 1285 - C2C12 Cell Lysate

NCBI Official Name

Tumor necrosis factor receptor superfamily, member 10b

NCBI Organism

Homo sapiens

Background Reference 01

Pan et al. Science 1997;277:815-8.

Background Reference 02

Sheridan et al. Science 1997;277:818-21.

Background Reference 03

Walczak et al. EMBO J 1997;16:5386-97.

Background Reference 04

MacFarlane et al. J Biol Chem 1997;272:25417-20

Citation 01

26009898

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18266105

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27827395

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11859410

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19570774

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23833311

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23319802

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21685476

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18846110

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20562908

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18193086

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18362888

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27387452

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30059709

Citation 20

24287696

Other Product Names

DR5 Antibody: DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5, DR5, UNQ160/PRO186, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TRAIL receptor 2