DR4 Antibody

Product Specifications

Background

DR4 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4) . The ligand for this novel death receptor has been identified and termed TRAIL2, 3, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1) . DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-κ B activation in many tissues and cells.

NCBI Gene ID

8797

Swiss Prot

O00220

Accession Number

AAC51226

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

1139

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KO Validation (Figure 1) : Anti-DR4 antibodies (1139) specificity was further verified by DR4 specific knockout cell lysate. DR4 signal was detected in wild type cells, but not in the DR4 knockout cells. KO Validation1139) Independent Antibody Validation in Cell lines (Figure 2) shows similar DR4 expression profile in human cell lines detected by two independent anti-DR4 antibodies that recognize different epitopes, 1139 against C-terminus domain and 1167 against the N-terminus domain. DR4 proteins are detected in all the tested cell lines except CaCo-2 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines1139 1167 KD validation (Figure 7, 10, 11) : Anti-DR4 antibody (1139) specificity was further verified by DR4 specific siRNA knockdown. DR4 signal in SW480, Huh7 and HeLa cells transfected with DR4 siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation1139

Field of Research

Apoptosis

Purification

DR4 Antibody is Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 - A431 Cell Lysate

Concentration

1 mg/mL

Buffer

DR4 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DR4 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

DR4 antibody has no cross reaction to DR5

Isoform

Human DR4 has only 1 isoform (468aa, 50kD) .

Symbol

TNFRSF10A

Positive Control 2

Cat. No. 1203 - A549 Cell Lysate

Positive Control 3

Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

Tumor necrosis factor receptor superfamily, member 10a

NCBI Organism

Homo sapiens

Background Reference 01

Pan et al. Science 1997;276:111-3

Background Reference 02

Wiley et al. Immunity 1995; 3:673-82

Background Reference 03

Pitti et al. J. Biol. Chem. 1996;271:12687-90

Background Reference 04

Schneider et al. Immunity 1997; 7:831-6

Citation 01

18193086

Citation 02

25032854

Citation 03

18511292

Citation 04

17470478

Citation 05

16007131

Citation 06

29020632

Citation 07

20562908

Citation 08

24980819

Citation 09

18594532

Citation 10

21941003

Citation 11

18599488

Citation 12

17212362

Citation 13

21835222

Citation 14

17522150

Citation 15

28529596

Citation 16

22784708

Citation 17

27406983

Citation 18

21372226

Citation 19

19414356

Citation 20

18680719

Other Product Names

DR4 Antibody: DR4, APO2, CD261, TRAILR1, TRAILR-1, DR4, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TRAIL receptor 1

Tested Applications

ELISA, WB, IHC-P, ICC, IF

Protein ID

1945072

Physical Properties

Liquid

Available Sizes

Curated Selection

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