TACE Antibody

TACE Antibody: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α . TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease) . TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) . TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

KO Validation (Figure 2) : Anti-TACE antibodies (1131) specificity was further verified by TACE specific knockout. TACE signal was not detected in TACE knockout HeLa cells as compared to that in control wild type cells. KO Validation1131) Independent Antibody Validation in Cell lines (Figure 3) shows similar TACE expression profile in human cell lines detected by two independent anti-TACE antibodies that recognize different epitopes, 1131 against C-terminus domain and 22-001 against a synthetic peptide to a sequence within amino acids 700-824 of human TACE. TACE proteins are detected in most of the tested cell lines except 293 and A549 cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines1131 KD validation (Figure 6,7) : Anti-TACE antibody (1131) specificity was verified by TACE TACE specific siRNA or shRNA knockdown. TACE signal in COS cells (Figure 6) and MDA-MB-435 cells (Figure 7) with TACE knockdown was disrupted in comparison with control. KD validation1131 Overexpression validation (Figure 8) : Anti-TACE antibody (1131) detected high expression levels of TACE in MCF-7 cells transfected with TACE as compared to vector transfected cells. Overexpression validation1131

TACE antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human TACE has 2 isoforms, including isoform A (824aa, 93kD), isoform B (694aa, 78.5kD) . This antibody detects only isoform A. Mouse TACE has 2 isoforms, long isoform (827aa, 93.1kD) and short isoform (655aa, 73.9kD) . Rat TACE has only one isoform identified so far (827aa, 93kD) .

TACE Antibody: CSVP, TACE, NISBD, ADAM18, CD156B, CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, ADAM 17