DOK1 Antibody

Product Specifications

Background

DOK1 Antibody: Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP) . p62dok represents a new family with very recently identified p56dok.

NCBI Gene ID

1796

Swiss Prot

Q99704

Accession Number

Q99704.1

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Cancer

Purification

DOK1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

1 mg/ml

Buffer

DOK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DOK1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

62 kDa

Fragment

IgG

Symbol

DOK1

Positive Control 2

Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 3

Cat. No. 1204 - K562 Cell Lysate

Positive Control 4

Cat. No. 17-004 - K-562 Cell Slide

NCBI Official Name

Docking protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R, Stillman B, Clarkson B p62 (dok) : a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 1997;88:197-204.

Background Reference 02

Yamanashi Y, Baltimore D Identification of the Abl- and rasGAP-associated 62 KDa protein as a docking protein, Dok. Cell 1997;88:205-211.

Background Reference 03

Holland SJ, Gale NW, Gish GD, Roth RA, Songyang Z, Cantley LC, Henkemeyer M, Yancopoulos GD, Pawson T. Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J 1997;16:3877-3888.

Background Reference 04

Di Cristofano A, Carpino N, Dunant N, Friedland G, Kobayashi R, Strife A, Wisniewski D, Clarkson B, Pandolfi PP, Resh MD. Molecular cloning and characterization of p56 (dok-2) defines a new family of RasGAP-binding proteins. J Biol Chem 1998;273:4827-4830.