Osteopontin (SPP1) (NM_001040060) Human Over-expression Lysate

Product Specifications

Overview

Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 3

Synonyms

BNSP; BSPI; ETA-1; OPN

Gene ID

6696

Accession Number mRNA

NM_001040060

Chromosomal Location

4q22.1

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

Field of Research

ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

Form

Liquid

Function

Druggable Genome, Secreted Protein

Molecular Weight

32.4 kDa

Storage Conditions

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Symbol

SPP1

Species

Human

Protein ID

NP_001035149

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.