What is the catalog number for NOTCH1 (NM_017617) Human Over-expression Lysate?

The catalog number for NOTCH1 (NM_017617) Human Over-expression Lysate is LH17914V.

What sizes are available for NOTCH1 (NM_017617) Human Over-expression Lysate?

NOTCH1 (NM_017617) Human Over-expression Lysate is available in the following size(s): 100 µg.

Is NOTCH1 (NM_017617) Human Over-expression Lysate in stock?

NOTCH1 (NM_017617) Human Over-expression Lysate is currently made to order / not held in immediate stock. Contact us for lead time.

NOTCH1 (NM_017617) Human Over-expression Lysate

Product Specifications

Overview

Transient overexpression lysate of Notch homolog 1, translocation-associated (Drosophila) (NOTCH1)

Synonyms

AOS5; AOVD1; hN1; TAN1

Gene ID

4851

Accession Number mRNA

NM_017617

Chromosomal Location

9q34.3

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Dorso-ventral axis formation, Notch signaling pathway, Prion diseases

Field of Research

Dorso-ventral axis formation, Notch signaling pathway, Prion diseases

Form

Liquid

Function

Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway

Molecular Weight

272.58 kDa

Storage Conditions

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Symbol

NOTCH1

Species

Human

Protein ID

NP_060087

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.