What is the catalog number for MYL9 (NM_181526) Human Over-expression Lysate?

The catalog number for MYL9 (NM_181526) Human Over-expression Lysate is LH15757V.

What sizes are available for MYL9 (NM_181526) Human Over-expression Lysate?

MYL9 (NM_181526) Human Over-expression Lysate is available in the following size(s): 100 µg.

Is MYL9 (NM_181526) Human Over-expression Lysate in stock?

MYL9 (NM_181526) Human Over-expression Lysate is currently made to order / not held in immediate stock. Contact us for lead time.

MYL9 (NM_181526) Human Over-expression Lysate

Product Specifications

Overview

Transient overexpression lysate of myosin, light chain 9, regulatory (MYL9), transcript variant 2

Synonyms

LC20; MLC-2C; MLC2; MMIHS4; MRLC1; MYRL2

Gene ID

10398

Accession Number mRNA

NM_181526

Chromosomal Location

20q11.23

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction, Vascular smooth muscle contraction

Field of Research

Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction, Vascular smooth muscle contraction

Form

Liquid

Molecular Weight

13.9 kDa

Storage Conditions

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Symbol

MYL9

Species

Human

Protein ID

NP_852667

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.