RNF146 (NM_030963) Human Over-expression Lysate
Product Specifications
Synonyms
DJ351K20.1
Gene ID
81847
Accession Number mRNA
NM_030963
Chromosomal Location
6q22.33
Expression System
HEK293T
Tag
C-Myc/DDK
Form
Liquid
Function
Druggable Genome
Molecular Weight
38.8 kDa
Storage Conditions
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Symbol
RNF146
Species
Human
Protein ID
NP_112225
Overview
Transient overexpression lysate of ring finger protein 146 (RNF146)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/81847
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_030963
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_112225
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