DNA Polymerase epsilon (POLE3) (NM_017443) Human Over-expression Lysate
Selected Variant
LH10262V
Size: 100 µg
Product Specifications
Overview
Transient overexpression lysate of polymerase (DNA directed), epsilon 3 (p17 subunit) (POLE3)
Synonyms
CHARAC17; CHRAC2; CHRAC17; p17; YBL1
Gene ID
54107
Accession Number mRNA
NM_017443
Chromosomal Location
9q32
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
Field of Research
Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
Form
Liquid
Molecular Weight
16.9 kDa
Storage Conditions
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Symbol
POLE3
Species
Human
Protein ID
NP_059139
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/54107
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_017443
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_059139
Price
Sign In for Pricing
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC042763-100 1x 100 µL
CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) (CD40LG/2763), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC471902-100 1x 100 µL
Nucleophosmin (Acute Myeloid Leukemia Marker) (NPM1/1902), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNCB1758-500 1x 500 µL
CELA3B / ELA3B (Pancreatic Function Marker) (CELA3B/1758), Biotin conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC040702-100 1x 100 µL
Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details