NECAP1 (NM_015509) Human Over-expression Lysate

Product Specifications

Overview

Transient overexpression lysate of NECAP endocytosis associated 1 (NECAP1), transcript variant 1

Synonyms

DEE21; EIEE21

Gene ID

25977

Accession Number mRNA

NM_015509

Chromosomal Location

12p13.31

Expression System

HEK293T

Tag

C-Myc/DDK

Form

Liquid

Molecular Weight

29.7 kDa

Storage Conditions

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Symbol

NECAP1

Species

Human

Protein ID

NP_056324

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.