14-3-3 theta (YWHAQ) (NM_006826) Human Over-expression Lysate
Product Specifications
Synonyms
1C5; 14-3-3; HS1
Gene ID
10971
Accession Number mRNA
NM_006826
Chromosomal Location
2p25.1
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection
Field of Research
Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection
Form
Liquid
Function
Druggable Genome
Molecular Weight
27.8 kDa
Storage Conditions
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Symbol
YWHAQ
Species
Human
Protein ID
NP_006817
Overview
Transient overexpression lysate of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/10971
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_006826
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_006817
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS6383437-01 0.1 mL
KAT5 (Histone Acetyltransferase KAT5, 60kD Tat-interactive Protein, Tip60, Histone Acetyltransferase HTATIP, HIV-1 Tat Interactive Protein, Lysine Acetyltransferase 5, cPLA(2)-interacting Protein, HTATIP, TIP60) (MaxLight 405)
Ask
View Details MBS6383437-02 5x 0.1 mL
KAT5 (Histone Acetyltransferase KAT5, 60kD Tat-interactive Protein, Tip60, Histone Acetyltransferase HTATIP, HIV-1 Tat Interactive Protein, Lysine Acetyltransferase 5, cPLA(2)-interacting Protein, HTATIP, TIP60) (MaxLight 405)
Ask
View Details