GRP78 (HSPA5) (NM_005347) Human Over-expression Lysate
Product Specifications
Synonyms
BIP; GRP78; HEL-S-89n; MIF2
Gene ID
3309
Accession Number mRNA
NM_005347
Chromosomal Location
9q33.3
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Antigen processing and presentation, Prion diseases
Field of Research
Antigen processing and presentation, Prion diseases
Form
Liquid
Function
Druggable Genome
Molecular Weight
72.3 kDa
Storage Conditions
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Symbol
HSPA5
Species
Human
Protein ID
NP_005338
Overview
Transient overexpression lysate of heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/3309
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_005347
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_005338
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