What is the catalog number for FLT3 (NM_004119) Human Over-expression Lysate?

The catalog number for FLT3 (NM_004119) Human Over-expression Lysate is LH07054V.

What sizes are available for FLT3 (NM_004119) Human Over-expression Lysate?

FLT3 (NM_004119) Human Over-expression Lysate is available in the following size(s): 100 µg.

Is FLT3 (NM_004119) Human Over-expression Lysate in stock?

FLT3 (NM_004119) Human Over-expression Lysate is currently made to order / not held in immediate stock. Contact us for lead time.

FLT3 (NM_004119) Human Over-expression Lysate

Product Specifications

Overview

Transient overexpression lysate of fms-related tyrosine kinase 3 (FLT3)

Synonyms

CD135; FLK-2; FLK2; STK1

Gene ID

2322

Accession Number mRNA

NM_004119

Chromosomal Location

13q12.2

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer

Field of Research

Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer

Form

Liquid

Function

Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane

Molecular Weight

112.7 kDa

Storage Conditions

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Symbol

FLT3

Species

Human

Protein ID

NP_004110

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.