Dystrophin (DMD) (NM_004016) Human Over-expression Lysate
Product Specifications
Synonyms
BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85
Gene ID
1756
Accession Number mRNA
NM_004016
Chromosomal Location
Xp21.2-p21.1
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Viral myocarditis
Field of Research
Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Viral myocarditis
Form
Liquid
Molecular Weight
72 kDa
Storage Conditions
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Symbol
DMD
Species
Human
Protein ID
NP_004007
Overview
Transient overexpression lysate of dystrophin (DMD), transcript variant Dp71b
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/1756
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_004016
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_004007
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
IHUCD300ARCFC6HISLY50UG 50 µg
Human CD300a Recombinant Protein C-Fc-6 His Tag Lyophilized 50UG
Ask
View Details 
MBS1232841-01 0.02 mg (E-Coli)
Recombinant Shigella boydii serotype 18 5'/3'-nucleotidase SurE (surE)
Ask
View Details MBS1232841-02 0.1 mg (E-Coli)
Recombinant Shigella boydii serotype 18 5'/3'-nucleotidase SurE (surE)
Ask
View Details MBS1232841-03 0.02 mg (Baculovirus)
Recombinant Shigella boydii serotype 18 5'/3'-nucleotidase SurE (surE)
Ask
View Details MBS1232841-04 0.02 mg (Mammalian-Cell)
Recombinant Shigella boydii serotype 18 5'/3'-nucleotidase SurE (surE)
Ask
View Details