What is the catalog number for ATP6V0E1 (NM_003945) Human Over-expression Lysate?

The catalog number for ATP6V0E1 (NM_003945) Human Over-expression Lysate is LH06954V.

What sizes are available for ATP6V0E1 (NM_003945) Human Over-expression Lysate?

ATP6V0E1 (NM_003945) Human Over-expression Lysate is available in the following size(s): 100 µg.

Is ATP6V0E1 (NM_003945) Human Over-expression Lysate in stock?

ATP6V0E1 (NM_003945) Human Over-expression Lysate is currently made to order / not held in immediate stock. Contact us for lead time.

ATP6V0E1 (NM_003945) Human Over-expression Lysate

Product Specifications

Overview

Transient overexpression lysate of ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1)

Synonyms

ATP6H; ATP6V0E; M9.2; Vma21; Vma21p

Gene ID

8992

Accession Number mRNA

NM_003945

Chromosomal Location

5q35.1

Expression System

HEK293T

Tag

C-Myc/DDK

Related Pathways

Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Field of Research

Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Form

Liquid

Function

Transmembrane

Molecular Weight

9.2 kDa

Storage Conditions

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Symbol

ATP6V0E1

Species

Human

Protein ID

NP_003936

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.