DBT (NM_001918) Human Over-expression Lysate
Product Specifications
Synonyms
BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2; BCOADC-E2; E2; E2B
Gene ID
1629
Accession Number mRNA
NM_001918
Chromosomal Location
1p21.2
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Metabolic pathways, Valine, leucine and isoleucine degradation
Field of Research
Metabolic pathways, Valine, leucine and isoleucine degradation
Form
Liquid
Function
Druggable Genome
Molecular Weight
53.5 kDa
Storage Conditions
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Symbol
DBT
Species
Human
Protein ID
NP_001909
Overview
Transient overexpression lysate of dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/1629
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001918
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001909
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