PFN3 (NM_001029886) Human Over-expression Lysate
Product Specifications
Synonyms
1700012P12Rik; profilin 3
Gene ID
345456
Accession Number mRNA
NM_001029886
Chromosomal Location
5q35.3
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Regulation of actin cytoskeleton
Field of Research
Regulation of actin cytoskeleton
Form
Liquid
Molecular Weight
14.6 kDa
Storage Conditions
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Symbol
PFN3
Species
Human
Protein ID
NP_001025057
Overview
Transient overexpression lysate of profilin 3 (PFN3)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/345456
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001029886
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001025057
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