PAH (NM_000277) Human Over-expression Lysate
Product Specifications
Synonyms
PH; PKU; PKU1
Gene ID
5053
Accession Number mRNA
NM_000277
Chromosomal Location
12q23.2
Expression System
HEK293T
Tag
C-Myc/DDK
Related Pathways
Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism
Field of Research
Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism
Form
Liquid
Function
Druggable Genome
Molecular Weight
51.8 kDa
Storage Conditions
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Symbol
PAH
Species
Human
Protein ID
NP_000268
Overview
Transient overexpression lysate of phenylalanine hydroxylase (PAH)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Leading Biology Transfection Reagent (TF002V) and 5ug Leading Biology GoldORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . Cell lysates were aliquoted and stored at -20°C before shipping.
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/5053
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_000277
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_000268
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