TEV protease Recombinant Protein
Product Specifications
Overview
Purified recombinant protein of TEV protease, with N-terminal HIS tag, expressed in E.Coli
Expression System
E. coli
Tag
N-His
Concentration
>50 ug/mL as determined by Bradford protein assay method.
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Activity
1 unit is defined as the amount of enzyme required to cleave 3 µg of a substrate protein to 85% completion in 1 hours at 30°C.
Form
Liquid
Buffer
25mM Tris, 150mM NaCl, 1mM EDTA, 5mM DTT, 50% glycerol, pH8.0
Molecular Weight
29.0 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
TEV protease
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